The application of innovative technology sgRNA-Click combined with CRISPR-dCas9

Figure 1. The CRISPR sgRNA was terminal uridylated with AMUTP utilizing TUTase enzyme. The azide-tailed sgRNA is recruited to gene of interest by dCas9. The localization of azide-tagged sgRNA to the target gene is visualized in cells as intense nuclear punctate dots (green).

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